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In brief,
In brief, expression plasmids were transfected into Vero cells (ATCC CCL81) using FuGENE6 transfection reagent (Roche).
In brief, expression was performed at room temperature for four hours after isopropyl-β-D-thiogalactopyranoside induction.
In brief, expression plasmid-carrying E. coli cells were cultured, induced, and harvested in 96 deep-well plates.
In brief, expression log2 ratio was calculated for individual genes, and ordered gene rank list was constructed for each of 281 batches (perturbagen versus vehicle pairs).
In brief, expression was binary graded for ER and PR, bcl-2, p21, Cyclin D1, and Ck 5/6, Ck 8/18 and SMA.
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In brief, the expression changes are ranked in a decreasing order (expression change vector).
We do not see similar changes with brief expression of HIF in the adult, but do find remarkable angiogenesis and ventricular dysfunction, which is readily reversible with cessation of transgene expression.
In brief, gene expression profiles were established from 80 frozen glioblastoma (grade IV astrocytoma), comprising 70 tumors from initial surgery, and 10 samples resected at recurrence, and from four non-neoplastic brain tissue samples.
In brief, for expression of opsin in COS-1 cells, 15 µg of plasmid DNA was used to transfect a plate (150×25 cm) of confluent COS-1 cells and the cells were harvested 50 56 hours post-transfection and washed two times with buffer A. The cell pellets were stored at −78°C.
In brief, TAA expression patterns could serve as important prognostic factors in HCC.
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