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Increasing the concentration of H2O2 results in an increase in APF fluorescence (Figure 1).
The mechanism for the hydrogen embrittlement of Ni-Ti superelastic alloy aged at room temperature after immersion in APF solution is dependent on the aging time.
Due to the variations in APF fluorescence and the presence of low concentrations of H2O2 in water the technique has an estimated lower detection limit around 50 nM hROS.
Therefore, the mechanism for the hydrogen embrittlement of Ni-Ti superelastic alloy aged at room temperature after immersion in APF solution was confirmed to be dependent on the aging time.
Fig. 4A demonstrates that the formation of peroxynitrite from SIN-1 was paralleled by an increase in APF oxidation at both, the physiological and basic pH.
As fluorescein is equally stable at pH values higher than 7.0 [53], the most rapid increase in APF oxidation at pH 9.0 is most likely due to the high stability of peroxynitrite.
Similar(53)
Cohn et al. [11] recently adapted the in-vitro APF method for use in pyrite slurries to quantify.
Overnight cultures of bacteria were diluted to the required concentration in APF-LB and lysed.
Overnight cultures of CVD 1920 pGEN2066) were diluted to biologically relevant concentrations (up to 103 CFU/ml) in APF-LB and 2 ml were lysed by microwave radiation.
Overnight cultures of S. Enteritidis CVD 1920 pGEN2066) were diluted to biologically relevant concentrations (up to 104 CFU/ml) in APF-LB and 2 ml were lysed by microwave radiation.
Sixteen Salmonella strains of various serovars were suspended in APF-LB media at 103 104 CFU/ml and 2 ml of each suspension were lysed using microwave radiation; every strain was lysed (93% ±16% mean ± standard deviation). 1 ml of the lysed sample was then assayed by MAMEF and a fluorescent signal was observed for every strain tested (Table 1).
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