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Microarrays were washed at low-stringent and high-stringent conditions and were stained with streptavidin phycoerythrin in accordance with established protocol.
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The cytotoxicity of the dual-gelled hydrogels was evaluated by a leachables extraction test, in accordance with established protocols.
All procedures were performed in accordance with established protocols and recommendations for the proper use and care of laboratory animals.
To prevent future outbreaks, it is vital that kids are inoculated in time and in accordance with established protocols by health centres and outposts.
Additionally, the DNA content of the digested samples was assessed with the use of bisbenzimide (Hoechst 33258; Sigma) in accordance with established protocols [ 20].
Total attachment loss was calculated as the sum of the gingival recession and the periodontal probing depth in accordance with established protocols [ 12, 16].
All tissues included were dissected and handled in accordance with established biobank protocols.
In keeping with routine management of patients within Improving Access to Psychological Therapies services, psychological wellbeing practitioners monitored patients' progress at each session, and delivery of care was structured in accordance with established stepped care protocols.
All procedures involving the sacrifice of the NCD and CD dogs and tissue handling were carried out in accordance with established animal use protocols at the University Health Network, University of Toronto, ON, Canada, and in compliance with the regulations of the Canadian Council on Animal Care.
Animal care was conducted in accordance with established guidelines, and all protocols were approved by the Midwestern University Institutional Animal Care and Use Committee.
Immunoablative therapy and HSCT (autologous and allogeneic) in AD is a highly complex procedure that should be done only in specialised centres in the context of approved study protocols in accordance with established guidelines [ 65].
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