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However, some questions still remain to be further investigated, such as in which tissues or cells these genes are silenced and whether the proposed receptor mediated endocytosis or the transmembrane channel-mediated uptake are the mechanisms leading to the production of RNAi effect as well as the pathway of RNAi effect leading to larval developmental stunt and death in ACB larvae.
In E strain ECB and in ACB, males do not use this enzyme for pheromone production.
We identified genes that are potential candidates for conferring Bt resistance in ACB.
This study identified differentially expressed unigenes related to general Bt resistance in ACB.
Presence of the two allelic classes in ACB could represent ancestral polymorphism.
To our knowledge, this is the first comparative transcriptome study to discover candidate genes involved in ACB Bt resistance.
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Cry1Ab exposure resulted in large alterations of the ACB transcriptome profile, including 636 unigenes being up-regulated and 3,157 being down-regulated in ACB-AbR.
Of these, 47 DEUs were down-regulated in ACB-AbR.
The increased proteolytic activity in ACB-AbR could reflect a higher ability to degrade toxins.
Compared to ACB-BtS, annotated proteases, chymotrypsin/trypsin, were over-transcribed in ACB-AbR.
Only one unigene (CL7354.Contig2) associated to ALP pathway was under-transcripted (−2.68) in ACB-AbR.
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