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Gene prediction and annotations were then conducted using Prodigal v.2.6.367 as implemented in Prokka v.1.1168 using the default parameters, or, as implemented in CheckM using the default parameters.
This process is completed by the program Helixturnhelix implemented in EMBOSS with the default parameters [ 19].
The putative coding sequence (CDS) of each PERNI transcripts of P. perniciosus was predicted using TransDecoder implemented in Trinity, with the default parameters setting.
Because the number of possible models is extremely large for the number of parameters we examine, we used a genetic algorithm to search the space of possible models, implemented in glmulti using the default parameters.
These log2 ratio signals were smoothed and segmented using Circular Binary Segmentation (CBS) algorithm [ 22] with the default parameters implemented in the DNAcopy R package.
MH haplotypes of each individual were inferred from degenerate sequence data using a Bayesian statistical method implemented in PHASE v.2.1 using the default parameters [ 58], an approach which allows the determination of allelic phase from degenerate electrophoretic profiles [ 59].
An InterPro Scan was also performed to find functional patterns and related GO terms by using the specific tool implemented in the Blast2GO software with the default parameters.
Subsequently, InterProScan [ 47] was performed to find functional motifs and related GO terms by using the specific tool implemented in the Blast2GO software with the default parameters.
Furthermore, InterPro Scan [ 45] was performed to find functional motifs and related GO terms by using the specific tool implemented in the Blast2GO software with the default parameters.
Analyses were performed with software implemented in the Galaxy analysis pipeline using the default parameters (Goecks et al. 2010).
The homology modeling was conducted using the default parameters of Prime v2.1 as implemented in Maestro 9.0.
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