In this study, we implemented a constraint-based sampling approach on an updated genome-scale network of yeast metabolism to systematically determine how EM level variations are linked to global changes in intracellular metabolic flux states.
In the present study, a new method to implement a strength constraint for the FBB method is proposed for the simultaneous optimization of more than two laminates (such as a panel and stiffeners).
Subsequently, an optimal fuzzy system (op-FS) is designed to fully implement a parameter constraint mechanism so as to guarantee the system stability converging to the desired state even if the UAD variability rate increases in a given range.
Here, we did not, but certainly can, implement a hard constraint to disallow any infeasible dihedral angles.
Because we implement a viability constraint that all genes must remain regulated, the effect of increasing the binding site length, at some point, is masked by the inability of pathways to lose any more binding sites.
While the underlying cellular objectives cannot be directly deciphered from these data, the changes in metabolite profiles can be implemented as a constraint to be satisfied, through formulation of a pseudo-reaction, grossly similar to the use of non-growth associated biomass objectives in bacteria [ 25].
Because the resultant sensitivity expression obtained by this procedure already compromises the effects of all the equality displacement constraints, a simple optimization method, such as the optimality criteria method, can then be used to implement an area constraint.
As explained in the main text, VS-SAIM's design is based on the principle of minimization of an energy function in order to implement a soft-constraint satisfaction.
We propose and implement an ∊-constraint method to jointly solve this TT and VS bi-objective problem.
Compared to the standard specification, the EGARCH framework exhibits several advantages namely: no need is imposed to artificially implement a non-negative constraint on the model parameters, while asymmetries are allowed under the EGARCH formulation.
