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The area was measured using digital images acquired via a CCD- camera and ImagePro 4.0 software.
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For double immunostaining, images were acquired via sequential scanning of each individual channel.
The images acquired by DW-MRI are constructed via quantifying the diffusion of water molecules in tissues and DWI uses differences in water motion to discriminate between tissues of varying cellularity [ 46, 47].
DIC images were acquired via illumination by a 100 W tungsten halogen and epi-fluorescent images were acquired via illumination by a 200 W metal halide lamp (Lumen 220, Prior Scientific, Rockland, MA).
Fluorescent images were acquired via epi-fluorescence microscopy, the numbers of fluorescence dots were counted one by one for quantification.
Images were acquired via scanning through the x-y-plane with 63 × objective lense.
Images were acquired via 1.1 NA 25× water-dipping objective (Nikon).
Images were acquired via a high-resolution CCD camera (Gatan, Pleasanton, CA, USA) and processed in DigitalMicrograph (Gatan).
The quality control images were acquired via the GenePix 4200AL scanner (Molecular Devices, CA, USA) at a 10 micron resolution and quantified with the Imagene 8.0 software suite.
Images were acquired via a JEOL-1530 Thermassistedisted field emission (TFE) Scanning electron microscope at 12 or 10 kV at the resolving powers indicated in the results.
In brief, T2-weighted images were acquired via a multi-slice turbo spin echo technique with a double inversion recovery pre-pulse for black-blood imaging.
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