Sentence examples for I wild from inspiring English sources

We investigated three different situations of limiting dNTPs: i) wild type cells incubated with hydroxyurea (HU), ii) cells with different concentrations of ectopically expressed mutant RNR protein, nrdB (D367G), and iii) cells with a severely impaired temperature-sensitive RNR protein, nrdA (L89P).

As shown (Figure 9B and 9C), NF-YA was recruited to the integrated (i) wild type or ChoRE-mutated (dmChoRE) promoters or the endogenous (e) Txnip promoter at similar efficacies under both the glucose-free and glucose-treated conditions, but not to the double CCAAT box-mutated (dmCAT) Txnip promoter, suggesting that the CCAAT box binding by NF-Y was not sensitive to glucose.

In this study, using a 'trapped' non-associating monomer and a non-dissociating dimer, we show that (i) wild type (WT) CXCL8 exists as both monomers and dimers, (ii) the in vivo recruitment profiles of the monomer, dimer, and WT are distinctly different, and (iii) the dimer is essential for initial robust recruitment and the WT is most active for sustained recruitment.

Following knockdown, we compared the migration of (i) wild type non-transduced AGS cells (ii) non-targeting scrambled shRNA transduced control cells and (iii) NET1 knockdown cells transduced with NET1 targeting shRNA (clone 65b).

(A ) Cross sections of hindlimb muscle from E14.5 (a – c ), E16.5 (d – f ) and E18.5 (g – i ) wild type (WT) and E18.5 Lrp4 –/– (j – l ) mice were double-labeled with anti-LRP4 antibodies (green) and Texas-Red conjugated α-bungarotoxin (red), which marks the site of the NMJ (arrowhead in a, d, g ).

The statistical significance of differences in parameters examined between: (i) wild types and corresponding mutants during CA and (ii) different time points of CA within the same strain were assessed with the one-way analysis of variance (ANOVA) with post hoc testing using a Dunnett's multiple comparison test.

This, however, is unlikely as i) wild-caught breeders did not show different longevities than lab-raised breeders in either sex and ii) breeders continued to have significantly higher longevities than non-breeders even after wild-caught breeders were excluded from the analysis (i.e. both the breeder and the non-breeder group consisted only of lab-raised animals).

Importantly, the non-cognate combinations of (i) wild-type telomerase and E215K TPP1 and (ii) K78E telomerase with wild-type TPP1 showed a similar reduction of RAP stimulation, to 78% and 68% of the full level, respectively.

Overall, our findings provide evidence that (i) wild-type p53 is not necessary to induce MDM2 transcription through the p53-responsive promoter (P2) and that (ii) other transcriptional factor(s) can regulate MDM2 mRNA expression through P2 in astrocytic gliomas that lack endogenous p53 protein expression.

The starting frequencies of the transgenic allele in permissive and restrictive rearing experiments were chosen to reflect those expected in extreme examples of two scenarios: (i) wild-type contamination in a mass-reared colony (≥0.5) and (ii) after cessation of inundative releases of fsRIDL insects into the field (0.25).

Conversely, standard curves were generated to verify that the RT PCR assays to detect: (i) wild-type miR-96, miR-96(+13G>A) or miR-96(+23A>G); (ii) wild-type miR-96* and miR-96* +66T>C miR-96* +66T>Cype pre-miii96, pre-miR-96(+42C>T) and pre-miR-96(+36T>C) had comparable amplification efficiencies (Supplementary Material, Fig. S2).