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The SAFs for the meso stress M i and for the temperature increment A i were determined by means of direct finite-element analysis of a micro-scale unit cell with proper boundary conditions.
Dissociation constant/inhibition constant (K i ) were determined by the interpretation of Dixon plot [ 11].
The amplitude-weighted lifetime is expressed as 6 The values of α i and τ i were determined using the PicoQuant Symphotime software with nonlinear least-squares fitting.
Changes in [Ca(2+)](i) were determined using microscopic imaging of fura-2 loaded Jurkat cells on poly-l-lysine-coated glass coverslips.
We eliminated all samples for which more than 5% of the SNPs were missing, and eliminated all SNPs that (i) were determined in fewer than 95% of the samples, (ii) had minor allele frequency less than 5%, or (iii) had a Hardy-Weinberg p-value of less than 10-6.
The fractional contribution of the ith component in the steady state is: (2) f i = α i τ i ∑ j α j τ j Individual values of α i and τ i were determined from simulation with PTI's Felix GX software with PowerFit 10 simulation module, using deconvolution of an instrument response function obtained from scattering and nonlinear least squares fitting to multiple exponentials.
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Absorption and desorption Pressure-composition-Isotherms (P-c-I) were determined between 23 and 80 °C to characterize their thermodynamic properties.
Serum concentrations of growth hormone and IGF-I were determined using ELISA kits from Shibayagi (Shibukawa, Gumma, Japan) and R&D Systems (Minneapolis, MN), respectively.
Fasting levels of total IGF-I were determined by RIA after acid ethanol extraction (Nichols Products Corp).
Antibodies against ML2028 (Ag85B) and ND-O-BSA, a synthetic analogue of phenolic glycolipid I (PGL-I), were determined as described [ 25].
Antigen levels of tissue plasminogen activator inhibitor (PAI-I) were determined from citrated plasma by immunoassay (ELISA) using kits (Biopool International, 6025 Nicolle St., Ventura, CA 93003).
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