Exact(3)
The real-time RT-PCR method utilizing SYBR Green I was optimized.
A real time PCR (RTm PCR) assay based on the detection with SYBR Green I was optimized in order to quantify the 1,2-dichloroethane dehalorespiring Desulfitobacterium dichloroeliminans strain DCA1.
The immunoprecipitation using sonicated whole cell nucleic acid, pre-treated with RNase I, was optimized, and tested using a Southern dot blot using a (TTAGGG n probe to confirm enrichment of the telomeric TERRA-associated R-loop [ 32] (Additional file 1: Figure S1g).
Similar(57)
I started programing during my bachelor's research project at the University of Cambridge, where I was optimizing the resolution of nuclear magnetic resonance structures of a particular protein, Ras.
The mechanical design and posture of Abigaille-III were optimized to reduce pitchback forces during vertical climbing.
Tree topology, the shape parameter of the gamma distribution of variable rates and, the proportion of invariable sites (I) were optimized during successive iterations.
To assess the validity of the absorption component of the model and its assumptions, the i.v. model was optimized to match accurately the observed i.v. data and the Caco-2 permeability, and aqueous solubility data were used to simulate the absorption.
The first 2.5 ml (1.0 ml sample volume and the first 1.5 ml) were discarded and the radiolabelled antibody was collected in the next 1.5 ml.In the first set, the I labelling efficiency was optimized by varying the amount of NaI present during the reaction.
Likewise, some of the patients who were not ED eligible were only not eligible because they were discharged on the day that all criteria were met (i.e., discharge was optimized).
The amount of RNase I and time of incubation was optimized for each sample based on the collapse of polyribosomes to the monosome peak as analyzed by analytical polyribosome gradients.
The gap placement was optimized iteratively.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com