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In this experiment, when 36 colonies were spread individually onto plates in the absence and presence of anhydrotetracycline, no difference in the number of colonies was observed (see Figure 2), suggesting that the intended cleavage of the genome by I-SceI was ineffective.
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While I think that the advice was well-intentioned, after spending years researching and writing Going Ape I have to say that I think that it was ineffective then and would be ineffective now.
This, combined with our observation that BIS-I is ineffective at both temperatures, suggests that basal PKC activity is low at both temperatures, but can be enhanced by application of a phorbol ester at physiological temperature.
"She might say that I'm ineffective," Mr. Seabrook said.
When I am sleep-deprived I'm ineffective, bitchy, off-balance.
The way I learned was ineffective.
In contrast, 4-ABAH (an inhibitor of MPO that interacts with Compound I [50]) was ineffective to modulate MPO-mediated peroxynitrite decay (Fig. 2A and B).
In media containing 6% ethanol, Tra1 was more disperse but the majority of the protein remained in foci, which we suggest are nuclear (DAPI staining was ineffective in this media).
Worse, it's ineffective.
"I thought he was ineffective," he said.
In animal with an SCI, such stimulation was ineffective, i.e. the ventilation response was abolished.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com