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The cultures dishes were prepared as follows; Rat tail collagen I was diluted in cold PBS to give a final concentration of 10 µg/ml and dishes were coated with 100 µl per cm2 dish area, resulting in a coating density of 10 µg/cm2.
SYBR Green I was diluted to the appropriate quantity using ddH20 before addition to the PCR master mix just prior to amplification reaction preparation.
Although Class I comprised the majority of all women studied, it is possible that the positive association between TG and Class I was diluted in the overall cohort, resulting in a weaker association.
The antibody directed against collagen type I was diluted 1 1000, the antibody against collagen type II was diluted 1 400, and the antibody against COMP was used at a 1 300 dilution in 1% bovine serum albumin in PBS.
As this inhibition is greatly enhanced by heparin, similar to the physiologic activation of AT-III by vessel wall heparan sulfate proteoglycans, AT-III was diluted in a buffer containing heparin.
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For activity measurements, SMP containing A- or D-forms of complex I were diluted to 1 mg/ml in PBS pH 7.4 and incubated with 8 μM of fluorescein-NHS (F-NHS) for 1 h at 10 °C in the dark.
For lysine labelling, SMP containing A- or D-forms of complex I were diluted in PBS pH 7.4 [24] to 10 mg/ml and incubated with 10 μM Cy5-NHS esters for 1 h at 10 °C.
For tyrosine labelling, SMP containing A- or D-forms of complex I were diluted to 5 mg/ml and treated with 2 to 15 μM of tetranitromethane (TNM) in SET buffer pH 8.0 prior to activity measurements or incubated with 3 mM N-acetylimidazole (NAI) in PBS pH 7.2.
SMP containing A- or D-forms of complex I were diluted to 1 mg/ml in PBS pH 7.4 and incubated for 1 h at 10 °C in the presence of 8 μM of either Cy3-NHS ester or Cy5-NHS ester.
Drinking just water all the way through a meal feels like I'm diluting what I'm putting in my mouth, as though I'm washing away all those nice flavours".
The I-Ang-I tracer was diluted in 0.1 M Tris to yield 18,000-22,000 CPM/mL, and 1 mL was added to each tube.
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