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(I ) Viability of H2.35 immortalized human hepatocytes treated with either scrambled, let-7a, or let-7b antiMiRs, measured at two and three days after transfection (n=10 each).
Apoptotic cell death was also determined by flow cytometry with an Annexin-V/PI viability assay.
We have recently developed a rapid and convenient SYBR Green I/PI viability assay amenable to high-throughput screening to identify new drugs targeting B. burgdorferi persisters (Feng et al., unpublished data).
To determine the type of cell death induced by the combination of CQ and the dual PI3K/mTOR inhibitors, cells were examined for apoptosis by assessing the Annexin V/PI viability assay, effector caspase 3 processing and cleavage of the caspase 3 substrate PARP.
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Thus we evaluated the effects of DON on (i) cellular viability and (ii) intestinal barrier integrity, using an in vitro model of the intestinal porcine epithelial cell line IPEC-J2.
We represent this phenotype as a binary vector of length 124 whose entry at position i indicates viability if sulfur source i is the sole sulfur source.
Thus, we examined the possibility of using of neonatal tissues instead of adult tissues because of some advantages: (i) higher viability of tissues, (ii) ease of slice preparation and (iii) significant signal levels even in L12 : D12 conditioned animals rather than long-day conditioned animals (Fig. 2C).
In order to examine the consequences of prolonged IGF-I stimulation, viability and clonal growth was examined in cultures exposed to IGF-I for 14 days.
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(I ) Cell viability after VSV infection as measured by trypan blue exclusion assay.
At higher concentrations of amphotericin B (>2 μg/mL) and DNase I, cell viability was reduced by 5 log10 units.
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