However, when damaged vessels are quantified, significant differences in the percentage of type I and II vessels are revealed; whilst type I vessels comprised approximately 80% of the vessel types in the wild type littermate controls, Cxcr2−/− animals displayed only approximately 10% type I vessels.
In terms of type I and type II damaged vessels, we observed no significant difference between the percentage of type I vessels between groups, however, we found significantly higher numbers of type II (more damaged) vessels in the bone marrow of the Cxcr2−/− animals compared to the wt littermate controls (c. 10% vs. c. 50% respectively; P ≤ 0·05).
A semiautomated computer-assisted program (Singapore I Vessel Assessment [SIVA], software version 3.0) was used to quantitatively measure the retinal vascular parameters from the photographs.
The software, known as Singapore I Vessel Assessment (SIVA), allows the measurement of a number of parameters from digital retinal images, including retinal vascular caliber and tortuosity [ 12].
Figures 1– 4 show a currently available semiautomated computer-based program, the Singapore I Vessel Assessment (SIVA), that measures a spectrum of retinal vascular parameters in addition to vascular caliber.
For measuring retinal microvascular geometric properties, right-eye retinal photographs of each patient were digitized and analyses were performed using a semiautomated computer-assisted image program (Singapore I Vessel Assessment or SIVA, Singapore).
The Singapore I Vessel Assessment 1.0 (Singapore) computer-assisted program was used to quantify parameters of retinal vascular network geometry from the field 1 image of the right eye of each individual.
Right-eye, digitized retinal photographs of each patient were analyzed by a single grader (M.B.S ., masked to participants' characteristics, using a semiautomated, computer-assisted image program (Singapore I Vessel Assessment or SIVA) as previously described (14, 15).
Right eye digitized retinal photographs of each patient were analyzed by a single grader, masked to participants' characteristics, using a semiautomated computer-assisted image program (Singapore I Vessel Assessment [SIVA], The National University of Singapore) as previously described (10).
Results were obtained for the following variables: (i) Total vessel density (TVD); the total numbers of vessels in the visual field.
"I'm a vessel, I'm a tool, sometimes a fool," riffed Yaw Agyeman, his voice silky and harmonic.
