Tcasα6, on the other hand, demonstrates a high degree of evolutionary conservation in RNA editing as it undergoes editing that alters two amino acid residues as is also the case for the orthologs of D. melanogaster, A. mellifera and the tobacco budworm, Heliothis virescens [ 33, 37].
Furthermore, in WA-CMS the ~1.1 kb transcripts do not undergo editing.
Most editing sites in the human transcriptome occur in clusters where a number of nearby sites undergo editing.
Figure 4 shows that not only was cDNA obtained from the Rafflesia RNA, suggesting that atp1 is expressed, it appears that the RNA undergoes editing because of the observed T at position 931 in the cDNA relative to C in the same position of the DNA sequence.
But the way it is recalled is sure to undergo editing.
The digitized vector data also have undergone editing, overlay analysis and topological checks.
However, although closely related, not every C T difference between M. polymorpha and P. endiviifolia coding regions reflects an actual editing site because only sites that are important for protein function seem to undergo editing.
In the case of tRNAIle AAU), which is reported to be edited (Auxilien et al, 1996), the Nrd1 CRAC recovered only RNAs that had failed to undergo editing of the anticodon loop (data not shown).
As the rest of uncoordinated clustered miRNAs seem not to undergo editing in the analyzed tissues, the impact of other mechanisms on the regulation of their expression remains to be revealed.
Third, in humans, about 6percentt of mature miRNAs undergo editing by the enzyme adenosine deaminase (Blow et al. 2006), resulting in alterations in either miRNA processing or miRNA efficiency (Kawahara et al. 2007; Yang et al. 2005).
Briefly, in the sequence chromatogram the Adenosine nucleotide that undergoes editing appears as a double peak: Adenosine for the unedited forms and Guanosine for those edited (the height of the two peaks was used for calculation of editing percentage) (Additional file 1: Figure S1).
