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Cells at 10% confluency were grown in TI or silibinin/TI supplemented medium for 24 h before treatment was removed and the cells were allowed to grow until confluence.
Whenever DNA and DNase I effects were tested, the medium was supplemented before biofilm seeding (t = 0).
In experiment 1, aliquots from ejaculates were supplemented before freezing with one of four levels of hyaluronan: 100 μg/mL, 200 μg/mL, 400 μg/mL, and 1000 μg/mL along with an untreated control.
The protective effect for pre-eclampsia may be greatest if L-arginine and antioxidant vitamins are supplemented before 24 weeks of gestation.
Can I submit an Optional Arts Supplement before I submit my application for admission?
2-Mercaptoethanol was added to a final concentration of 0.352% to supplemented media before each use.
It was losing money, which meant I had to supplement my work elsewhere before I was eventually made redundant.
Beads were washed 3 times in 1 ml of supplemented IP-Buffer before use.
Absolute live cell counts were obtained by supplementing cells before flow cytometric analysis with CountBright Absolute Counting Beads Life Technologiess) and acquiring each sample based on bead count.
It encumbers the dialogue about supplements, too, where the reality is more nuanced than panacea vs. B.S. I have written at length about nutrient supplements before, and won't do so again here; those prior columns are at your disposal.
I work plenty and that could have helped supplement my income before my new salaries in place.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com