Sentence examples for I supplement with from inspiring English sources

Cape Cod has an ideal climate for tomatoes — sunny days and the right soil (acidic, nitrogenous, and well-drained), which I supplement with fertilizer and hand-watering.

I have a pretty good balanced diet, a mix of protein and carbs, and I supplement with hydration, with salts, so as not to cramp.

(i) Supplement with collaborative networks Internet searches for working groups and collaborative networks related to older adults' mobility and the built and the social environment further facilitated the identification of specific stakeholder groups.

I am paid a living wage (full disclosure: I supplement with grants).

A431-CCK2R cells were grown in Dulbecells Modified Eagle medium were grownMAX-insupplementeDulbecco0 % (v/v) fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin, 4500 mg/L D-glucose, and 250 μg/mL G418.

Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing glutamax-I supplemented with 10% fetal calf serum and 1% non-essential amino acids (PAA, Cölbe, Germany) at 37°C in a humidified atmosphere containing 10% CO2 (pH 7.4).

The human hepatoma cell lines HuH7.5 (Apath, L.L.C., St.Louis, MO) and HepG2 (Prof N. Maitland, University of York, York, UK) were cultured in DMEM with Glutamax-I, supplemented with 10% FBS, 1 mM sodium puruvate, 3.2 mM L-glutamine, 1% Non-Essential Amino Acids, 1% PEST.

Once they reached ∼70% confluence, they were rinsed with PBS and kept for an additional 24 hrs in serum-deprived media, which is composed of DMEM+GlutaMAX™-I supplemented with fatty acid free bovine serum albumin (0.1%, BSA, Sigma-Aldrich, St . Louis MO) and insulin-transferin-sodium selenite liquid media (1×, ITS, Sigma-Aldrich).

All cell lines were cultured in DMEM/glutamax-I supplemented with 10% heat-inactivated FCS and antibiotics (Life Technologies, Cergy Pontoise, France).

mMSCs (Invitrogen, Carlsbad, CA, USA), which are produced from bone marrow isolated from C57BL/6 mice, were maintained in DMEM-F12 with GlutaMAX-I supplemented with 10% MSC-qualified FBS (Invitrogen).

After co-transfection, cells were grown in DMEM Glutamax-I supplementeDMEM Glutamax-Il bovine supplementediotic-Antimycotic and after 24 h the stable integration withgradually selected using 10% μg/ml ofetalromycin bovineivogen).