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And the presence of apoptotic markers was analyzed with annexin V-FITC/PI stains.
A comparison of the phylogenetic grouping and temporal distribution of the 74 PEDV strains from North America showed that clade I mostly consisted of strains collected between November and December 2013 (n = 38); only 5 clade I stains were collected during July October 2013 (Table).
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(i) Stain Preparation Procedure.
Mitochondrial transmembrane potential was evaluated by JC-I staining.
Specific apoA-I staining was present in all samples.
The nuclei become visible as dark spots in the Troponin I-stained cardiomyocytes.
To visualize changes in fibrosis in the kidney interstitium, collagen-I staining was performed.
The correlation coefficient between the assessment of Id staining by two independent observers was r = 0.93 0.96.
Surface MHC-I staining was analyzed by flow cytometry on a FACS-Calibur (Becton Dickinson, UK).
I noticed stains I hadn't seen before.
Apoptosis was determined by Annexin V/DAPI staining.
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