Total RNA (10 μg) was extracted from three different cell populations: i) sorted TRH-GFP+ cells (GFP+); ii) TRH-GFP+ and GFP- mixed cells (GFP+/-) passed through the FACS but not sorted, and iii) non-transfected cells (NT).
To account for the effect of differential gene expression on its probability to be an outlier we first i) sorted all genes according to –LOG(P) from largest to smallest, and for each known cancer gene, and then ii) took non-cancer genes from the list immediately above and below of a given known cancer genes.
Back in New York, I sorted through my notes on the men's personal tragedies.
As I sorted through them, I came across a faded velvet photograph album.
These memories came flooding back as I sorted through my make-up box.
The man overseeing the estate sale approached me as I sorted through them.
Here's what I did when I got back to New York: I sorted my takeout menus.
"I sorted them out and had a good cry," he told the University of Chicago alumni magazine in 1997.
I sorted through some of the varieties available, planting, growing and evaluating flavors of the reputed best.
"He had a bit of an attitude on him so I sorted that out," said Jones, matter-of-factly.
As I sorted through my gear, I met Lee Van Alen Manigault, another member of our team.
