Exact(33)
The cell pellet was resuspended in 400 µl of Cytoplasmic Extraction Reagent I solution containing protease inhibitors.
A promising approach for conductive patterns with high efficiency for flexible electronics was developed by direct-writing, silver(I) solution (silver nitrate, acetate silver, etc).
Two microlitre of DNase I solution (concentration series) or serum samples were added to the wells.
Slowly the supernatant was removed and to the pellet added 100 μl fresh ice-cold Otto I solution.
Briefly, tracheas were digested with dispase I solution (2.5 U/ml dispase I, Roche) for 2 h at 37°C.
In brief, small pieces of tissues were fixed in ice cold (2°C) HOPE I solution for 1 d to 3 d.
Similar(27)
For the in-vitro experiments, 100 µL of RADA16-I solution containing EGF were put directly on top of the wound.
When all peptide solutions are mixed 1∶1 with RADA16-I solution (1% w/v), they form highly viscous gel solutions.
Cone voltage was optimized by infusion of 1 μg/mL standard AA-I solution in methanol.
Alternatively, a commercially available rat-tail collagen type-I solution was used (BD Biosciences, Bedford, MA, USA) containing 4.01 mg/mL total protein in 0.02 N acetic acid.
Unless otherwise indicated, experiments were conducted on tissue culture plastic substrata coated with 0.1mg/ml collagen-I solution (Millipore) overnight at 4°C.
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