Sentence examples for I site on from inspiring English sources

The resulting fragment was ligated to the Sph I site on a cDNA fragment 1173-stopp).

A 1064bp DNA fragment in mouse Wnt11 promoter proximal region was cloned into XhoI/HindIII sites on pGL3-basic plasmid.

Using PCR, the TAS-gfaABC1D enhancer and promoter was amplified and isolated with AscI/SalI sites on the 5' and 3' ends, respectively.

The 3'-side of Aaku80 digested with Pac I, and Xba I was ligated to the Pac I and Xba I sites on pCO5P to yield pCO5P3.

In pBD003, the Kpn I and Nhe I sites on the vector can be used for other viral infectious clone constructions.

Among them, 78,548 probes target 42,978 MspI/HpaII sites on chr18, with 35,570 sites targeted by a matching pair of probes.

Then the promoter fragment was cloned into Kpn I/ Nco I sites on pCAMBIA 1302 by substitution of 35S promoter in the front of GFP gene.

A DNA oligomer called TTC17, CAACCATGGTCTCG TTC 17 GTCTTCCTAGGAAC, was synthesized with 17 repeats of the TTC triplet in the center, a BsaI site on the 5′ end and dual BbsI-AvrII sites on the 3′ end.

The primers used for PCR amplification were 5′-GGGGTACCGGAGTCATGAGGGCGAACGACGCTC −3′ and 5′-ATAAGAATGCGGCCGCGTTCTCTGGGCATTAGCCTTGGG −3′, and the HER3 fragment was cloned into the Kpn I and Not I sites on the vector.

The enzyme cutting efficiency (35% on control cells and 33% on SETD2-depleted cells) was determined by real-time quantitative PCR (RT-qPCR) with primers flanking the I-SceI cleavage site on the GFP reporter gene.

"When I arrived on site on Sunday at around 7am I said to myself how dangerous those barriers are because they are not protecting the spectators.