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The second sum is over the nearest neighbors of i site.
The complex I site producing O2− during RET is unclear [ 85, 97].
Above, a DNAP η mechanism was discussed for extracting the 3′-OH proton (i site).
Cu(I) site is more efficient with respect to both processes than Ag(I) one.
The primers are as follows: 5'-TTGGATCC andGATATGATGGAGGAGGA-3' and 5'-TCACTCGAGGAACTTGTGCCACTGATGATCA-3' with an underlined BamH I site and Sal I site, respectively.
This fragment was then cloned into the Sph I site of pUC118 (Takara) to yield pAaku80.
B (Bgl II site), S (Sph I site), EXT (external probe), INT (internal probe).
A transcript with an engineered Xho I site from two ligated PCR fragments amplified from pJEV-1 was also infectious.
VX-222 and ANA598 are two NNIs targeting thumb II site and palm I site of HCV NS5B polymerase, respectively.
The underlined nucleotides mark the Kpn I site.
PCR primers were GCTCTAGA-TGGGTATCGTGTTAATATCAG (forward primer with Xba I site) and GCGGTACC-ACAGCGTGGCGAAGTGCGTA (reverse primer with Kpn I site).
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