These patients were: (i) sequenced for the USH2A c.7595-2144 A>G, p.Lys2532Thrfs*56 change, which causes inclusion of a pseudoexon; and (ii) screened using MLPA to detect deletions and duplications in the USH2A gene.
At each iteration i the algorithm constructs the so called i-traces for the given permutations π and π T, that are the traces that contain all the optimal i-sequences for sorting π into π T. The i-traces are constructed from the previous (i - 1 -traces with the following procedure.
All suppressors that mapped to Chromosome I were sequenced for intragenic suppressor mutations in the rsa-1 gene.
Let s = ρ1 ρ2... ρ i be a valid i-sequence of reversals for a permutation π.
(1) Rainville, I, et al. Next-Generation Sequencing for Inherited Breast Cancer Risk: Counseling Through Complexity.
I sequenced multiple haplotypes for one mitochondrial locus and 13 nuclear loci from the four species using primers and methods described previously [ 24, 33].
Cytochrome oxidase I (COI) was sequenced for woodpecker species for which cytochrome b (cyt b) sequences were available.
Parts of the mitochondrial genes for cytochrome oxidase I (COI) were sequenced for each sample.
αi in turn is estimated as the sum of nucleotide (i) in all strains sequenced for that locus.
This demonstrates the desirability of true biological replicates (i.e., transcriptomes sequenced for each individual) to distinguish actual responses to a treatment from individual variation.
The two mtDNA hypervariable regions, HVS-I and HVS-II were sequenced for nucleotide ranges: 16024 16365 and 72 340, following previously described protocols [23].
