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These patients were: (i) sequenced for the USH2A c.7595-2144 A>G, p.Lys2532Thrfs*56 change, which causes inclusion of a pseudoexon; and (ii) screened using MLPA to detect deletions and duplications in the USH2A gene.
With sequencing data, we will not observe the true allele frequencies P i, but rather the m i sequenced fragments that overlap the location i, of which X i of them are mutated.
Working among the vestiges of Fred Sanger's empire, I sequenced the DNA and mapped the mRNA transcripts of a segment of the human cytomegalovirus genome and discovered the thrill of working all hours in the company of like-minded science addicts.
Springsteen felt The River show succeeded, saying "I sequenced [the album] to feel like a live show, so you have four fast songs and a couple of ballads.
For these next experiments, I sequenced all pha-1 (ts) rescued F1's.
In addition, I sequenced an ancestor sample consisting of DNA from pooled g1 worms of all three replicates.
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The nine-residue sequence is entirely conserved in all Group I sequences examined.
However, there is strong nodal support for the monophyly of all squamate class I sequences (A.
V1R I sequences originating from Sanger sequencing of cloned amplicons [ 29].
Additional file 2: Dataset 2 contains filtered V1R I sequences originating from Yoder et al. [ 29].
The topoisomerase I sequences sufficient to bind BTBD2 were mapped to residues 215 to 329.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com