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In this model, the ith device is represented by a collection m i of n i relevant parameters (1).
The above results consist of (i) relevant non-linear (elastic and elastic-plastic) equilibrium paths, (ii) deformed configuration evolutions along those paths, evidencing D-G interactive effects, and (iii) figures providing the failure mode characterisation.
At each generation, the quantities d i, and/or E i relevant in each landscape are evaluated.
Hidden Markov Models (HMM) for (i) relevant proteins (i.e., β-galactosidases, bacteriocins, glycosyl hydrolases) and (ii) proteins previously shown to be involved in relevant phenotypes (i.e., synthesis of non-ribosomal peptide antibiotics) were obtained from Pfam 26.0 protein families' database [ 87] (see Additional file 13 for Pfam models used for the searches).
In general, tracking procedures consist of two steps: (i) relevant objects are segmented from the background in each frame by using, for example, the watershed algorithm (Grau et al., 2004; Malpica et al., 1997; Vincent and Soille, 1991), and (ii) each of the segmented objects is then linked to the nearest object in the subsequent frame (Hadjidemetriou et al., 2004; Meijering et al., 2006).
Absolute time was not used because (i) relevant fossil data are not available to calibrate dates in the phylogeny and (ii) the number of generations per year is assumed to differ dramatically between nematode species due to differences in generation time and, given ecological differences (Herrmann et al., 2006; Kiontke et al., 2011), presumed lengths of diapause (dauer) stages.
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