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We develop heuristic methods with performance guarantees using three approaches: (i) relaxation rounding, (ii) water filling, and (iii) greedy packing.
Stage I relaxation, as measured by percentage of the original stress relaxed, accounts for at least 50% of the total stress relaxed despite occurring in only 5 10 min and was attributed to lamella sliding and compaction, and permanent intralamellar crack closure (for tests conducted at higher temperatures).
A DNA sequence-dependent nucleosome structural and dynamic polymorphism was recently uncovered through topoisomerase I relaxation of mononucleosomes on two homologous ∼350 370 bp DNA minicircle series, one originating from pBR322, the other from the 5 S nucleosome positioning sequence.
The combilexins were also evaluated for DNA binding by measuring the increase in DNA melting temperature, for DNA topoisomerase IIα-mediated double strand cleavage of DNA, for inhibition of DNA topoisomerase IIα decatenation activity, and for inhibition of DNA topoisomerase I relaxation of DNA.
Topo I activity was measured using the Topo I relaxation activity kit (www.Topogen.com).
In our study, the majority of subjects with IR and IGT had a mild form of LVDD (grade I, relaxation abnormalities).
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I must admit that I find relaxation something of a strain - especially when it's enforced.
When I met the pre-prep headteacher for interview I was grilled on why I taught relaxation.
One arbitrary unit of topoisomerase I activity was defined as the amount of topoisomerase I showing relaxation of 0.25 μg DNA under the above-described conditions.
"Is muscle relaxation dangerous?" asked one.
Is this relaxation process slower or faster than cell cycling?
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