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Results were summarized in a contingency table, where TFBSs were positively counted when found significantly enriched for each method.
And you will count positively what flows toward that side and negatively what flows towards the other side.
For GFP expression analysis, 2D gating was used to separate increased autofluorescence signals from increased GFP signals to more accurately count positively expressing cells.
For IHC quantification, NIH ImageJ [ 28] was used with a cell counter plug-in to manually count positively stained mammary epithelial cells vs. total epithelial cells in multiple fields.
Similarly, expression of p16 (CDKN2A), pRb (RB1) and p53 were scored by counting positively staining nuclei, i.e., Null/Weak 0 (<10%), Intermediate 1+ (10 25%), 2+ (25 50%) and Strong 3+ (>50%).
The percentage of positive cells was calculated by counting positively stained cells in the randomly chosen fields.
Due to the relatively small volume of ED2/CD163 positive cells, this marker was quantified by counting positively stained cells on an Olympus fluorescence microscope.
Let (text {VM}^{i}_{t}) denote the corresponding variation margin, counted positively when received by the bank.
Keep that department store credit line you opened 10 years ago, because it counts positively toward your credit score.
Data were obtained by manually counting positively stained cells in ten separate fields under 400× high power magnification.
The weights (cg,r) were calculated by totaling up matches (counted positively) and mis-matches (counted negatively) and dividing by the number of possible states for a given GPR.
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