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Recordings were made 3 6 days following surgery while patients were seated on a bed, resting following (i) overnight withdrawal of antiparkinsonian medication, termed OFF drug; and (ii) ∼1 h after 200 mg levodopa administration, termed ON drug.
The recovered fraction was then subjected to two dialysis at 4 °C: (i) overnight against 20 m Tris-HCl, pH 8, 150 m NaCl, 4 urea; and (ii) for 3 h against buffer B (20 m Tris-HCl, pH 8, 50 m NaCl, 2 urea, 0.1 m CaCl2).
In order to improve the quality of extractions, we modified a few steps: (i) overnight lysis was conducted for most samples, (ii) elution was performed twice using 100 μl of Buffer AE and finally (iii) incubation time was extended to 5 min so that a higher concentration of DNA was obtained.
I get up early in the morning and I work all the way through and I overnight flight back.
She was giddy with excitement to see it - and asked that I overnight her a DVD.
Precipitated DNA was dissolved in water and digested with 50 units of Sca I overnight.
Similar(48)
I have got a couple of raises since I started, and since I work overnight I get $1 more.
I read it overnight.
I read it overnight and emailed Sally the next day".
"I learnt it overnight and conducted it the next day.
"I think I want to stay overnight," I say.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com