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Primary antibodies were used as follows: (i) mouse monoclonal 6D6 (Aviva System Biology, USA; dilution 1∶2000) raised against morphine-like compounds (morphine, morphine-3-glucuronide, and M6G, based on supplier specifications and our own experiments) and (ii) rabbit anti-lactoferrin polyclonal antibody (dilution 1∶5000) [33].
Let E i (human) and E i (mouse) be the codons encoding amino acid D i (human) and D i (mouse) in the corresponding mRNA sequences.
Membranes were probed with primary antibodies, either (i) mouse monoclonal anti-GFP (Roche), (ii) anti-V5 or (iii) anti-2A antibodies.
For dual immunofluorescence labelling, the sections were incubated for 48 h at room temperature in a primary antibody cocktail containing: (i) mouse anti-GS (1 1000 dilution) and (ii) rabbit anti-GFAP (1 5000 dilution) simultaneously.
The sections were incubated for 48 h at room temperature in primary antibody cocktail containing: (i) mouse anti- β amyloid monoclonal antibody (A β; 1 : 2000; Covance) and (ii) rabbit anti-SERT polyclonal antibody (1 : 2500, Immunostar) simultaneously.
We employed two cell models to study osteoblast mechanosensitivity: i) mouse bone marrow cells cultured in the presence of ascorbic acid (50 mg/ml) for 4 6 days or ii) C2C12 cells stably transfected with BMP-2 and cultured for 2 6 days.
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(i) mice drank glucose; and (ii) drank glucose plus doxycycline in water.
Is mouse girl wearing actual Christopher Kane?
To determine whether DCN could play similar pro-tumoural functions in human bladder tumours as in the MB49-I mouse model, we first analysed expression of DCN mRNA in human bladder carcinoma cell lines.
To study CD8+ T cell interaction with the spinal cord microvasculature during EAE we have therefore decided to first investigate CD8+ T cells from a TCR transgenic OT-I mouse.
BM-DCs transduced with IiOVA-GFP or IiOVA-p5-GFP activated profiferatiOVA-specificeCD4ic CD4 and CD8 T cells purified from transgenic OT-II and OT-I mouse strains, respectively (results not shown).
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