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It was reported that H2O2 activates phospholipase C on VSMC through tyrosine phosphorylation and that this activation has a major role in rapid [Ca2+] i mobilization in this type of cells [ 135].
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Moreover, similar agonist-induced desensitization of [Ca2+]i mobilization and IP3 generation occurred when I-BOP was used as the TP-stimulatory ligand (data not shown).
Changes in [Ca2+]i mobilization were determined by measuring peak rises in [Ca2+]i mobilized (Δ[Ca2+]i) and were calculated as mean changes in Δ[Ca2+]i ± S.E.M. (nM).
U46619-stimulation of TPαΔ336, a truncated variant of TPα eliminating two potential target phosphorylation sites at Thr and Ser, yielded efficient [Ca2+]i mobilization and IP3 generation in response to U46619 (Fig. 5G and I, respectively).
Specifically, the level of [Ca2+]i mobilization by TPαS331A following secondary stimulation corresponded to 39% of that mobilized following primary U46619 stimulation.
Pre-stimulation of TPαΔ336 with U46619 impaired but did not abolish [Ca2+]i mobilization and IP3 generation in response to its secondary agonist stimulation (Fig. 5H and I, respectively).
2-OMe-LPC possessing in sn-1 position the residues of myristic, palmitic, stearic and oleic acid were also evaluated as factors regulating [Ca2 +]i mobilization and cAMP levels.
However, as shown in Figure 3A and B, the [Ca2+]i mobilization induced by both BK and TG was not associated with any detectable peroxynitrite production.
Using Ca2+ imaging, we observed that physiologically appropriate concentrations of OXT evoked [Ca2+]i mobilization in a subset of taste cells (EC50 ∼33 nM).
These results further supported that the LA-induced [Ca2+]i mobilization was associated with peroxynitrite generation and the latter leading to cellular protein nitrotyrosylation in HM cells.
As shown in Figure 2C and D, LA-induced [Ca2+]i mobilization was associated with the generation of superoxide and NO.
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