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However, our data establish that residual complex I measured using the 20-kDa subunit as a marker correlates with the residual complex I activity in affected tissues.
A wild-type induction profile Z reg WT (I ), measured using the fluorogenic substrate FDG (see Materials and Methods), is shown in Figure 1c.
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The resulting changes in [Ca2+] i were measured using CASMs.
Cytosolic free Ca2+ levels ([Ca2+] i ) were measured using fura-2 as a probe.
Changes in [Ca2+] i were measured using the Ca2+-sensitive fluorescence ratio dye Fura-2 as described previously (Dingemans et al. 2007, 2008).
[Ca2+] i was measured using a fluorescent plate reader (Varioskan Flash, Thermo) at 510 nm emission, with excitation wavelengths of 340 nm and 380 nm.
Serum levels of IGF-I were measured using retro-orbital blood and the Mouse/Rat IGF-I kit from Diagnostic Systems Laboratories.
These results suggest that MC-I activity measured using 18F-BCPP-BF is more sensitive than conventional biochemical and histological parameters.
The numbers of pixels of each area as well as the numbers of OT-I were measured using ImageJ software (National Institutes of Health).
Urine CTX-I was measured using the Nordic Bioscience Diagnostics™ serum CrossLaps® ELISA assay kit (Denmark).
The troponin I was measured using an immunofluorescent assay calibrated with spiked serum (Biosite Inc., San Diego, California).
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