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All qPCR were carried out using LightCycler® 480 SYBR Green I Master on Roche LightCycler® 480 instrument following the manufacturer's guidelines and cycling conditions.
All qPCR were performed using LightCycler 480 SYBR Green I Master on Roche LightCycler 480 instrument following the manufacturer's guidelines and cycling conditions.
In samples from knock-out livers, analyses were performed using LightCycler® 480 SYBR Green I Master on LightCycler® 480 (Roche Diagnostics GmbH, Mannheim, Germany), except Cyp2b10, which was done as described above.
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Real-time quantitative PCR (RT-qPCR) was performed using the LC 480 Sybr Green I master kit on a LightCycler® 480 Real-Time PCR system (both from Roche Applied Science, Penzberg, Germany).
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The cDNA was analysed by quantitative PCR with SYBR Green I master (Roche) on a LightCycler 480 (Roche).
cDNA was synthesized using Transcriptor First Strand cDNA Synthesis (Roche, Switzerland), and qPCR was performed using SYBR Green I master mix (Roche) on a Roche LightCycler 480 (Roche).
The reaction was diluted to a final volume of 50 μl and 1 μl cDNA was used as template for PCR using LightCycler 480 SYBR Green I master mix (Roche Diagnostics) on a LightCycler 480 (Roche Diagnostics) in triplicate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com