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DNA sequencing of a single copy nuclear ribosomal protein S9 and the mitochondrial cytochrome oxidase I loci were then investigated and 12 nuclear microsatellite markers were developed and analysed.
Further we also found susceptibility/protective alleles at class I loci, and other class II alleles.
Initially the association between HLA and MS was studied in HLA Class I loci [12].
These studies are therefore unable to address the differential expression of HLA class I loci [13].
The basic structure of class I loci is highly conserved across vertebrates.
Genotyping at HLA class I loci was performed by sequence specific primers (SSP) PCR as described elsewhere [52].
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Ombrello et al. depicted an association of the MHC-I loci and BD [53].
An accuracy of 95% was shown to be achievable at 10× average coverage depth on HLA-I loci (Fig. 3).
PCR was utilised to profile the presence of these AES-I loci in a total of 188 P. aeruginosa strains.
To tackle these issues, we developed a new method named OptiType, which considers all major and minor HLA-I loci simultaneously.
Pseudomonas spp. produce at least two distinct AHL signal molecules from the lasR-I and rhlR-I loci, which are hierarchically controlled and work in concert to control virulence and biofilm formation.
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Justyna Jupowicz-Kozak
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