Exact(9)
In the presence of the excessive amount of 6.7 nmol NaI the I labelling yield was 96% upon using the same amount of L19-SIP (100 μg, 1.33 nmol) and the same amount (25 μg vial-coated) IODO-GEN.
To explicitly model these different dynamic regimes, and the behaviour of mtDNA copy number during each, we include six different dynamic phases throughout development, each with different rates of replication and degradation (labelled with subscript i labelling the dynamic phase: hence λ1, ν1,…, λ6, ν6), and allowing for different rates of cell division or quiescence.
As convincingly shown by Fig. 1, addition of NaI as carrier to the reaction mixture during I labelling provides a means to increase the labelling yield with this precious isotope.
Table 1 Technical protocol 125 I labelling of BoNT-A according to IODOGEN-coated method Step 1.
The MAb cetuximab (Erbitux® conc. 2.0 mg/ml) was used as reference for optimization of I labelling and was purchased from Merck, Darmstadt, Germany.
The Roche LightCycler Real-time PCR system (Roche Diagnostics Corporation, Roche Applied Science, Indianapolis, IN, USA) was used to quantify the expression of target genes with SYBR Green I labelling.
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