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Equation (5) gives the distance of the pixel to the contour, where μ is a constant that indicates the distance coefficient s i g n(s i,Ω =1 when s i is inside Ω and s i g n(s i,Ω)=−1 when s i is outside Ω; and l o c(s) is the coordinate position of pixel s in the image.
Delta_{w}:P i,C >T_{p} cap S i >T_{s} where Op i,o) denotes the probability of each node i belong to the object o, P i,C) is raw probability of category C, S i) is the saliency value of i. ib i,o) indicates whether i is inside of the scope of o, ib i,o)∈{0,1}.
Primer sequences used in this work (the underlined sequences indicate restriction sites, the restriction site of BstP I is inside the PCR product) are listed in Table 3. Pdr5p-GFP protein fusions were constructed as previous described[55].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com