Exact(2)
In a TechCrunch poll I inserted into that post (and again below), 77percentt of you agreed.
In addition, the Federal Reserve provided over $16 trillion in total financial assistance to these same institutions during the financial crisis (which only became public after an amendment I inserted into the Dodd-Frank Wall Street Reform and Consumer Protection Act requiring the Fed to disclose this information).
Similar(58)
Both amplicons were cloned as BglII-NotI or BamHI-NotI inserts into pPIC9 digested with BamHI and NotI resulting in plasmids pPIC9-cbhII and pPIC9-eglII.
cDNAs were cloned as BamHI/NotI inserts into the multiple cloning site of pcDNA5 FRT/TO that adds a C-terminal GFP tag to the expressed protein.
The TKNMI library was used in combination with the pre-existing CHORI-260 library (EcoRI inserts into pTARBAC2.1, average insert size of ~190 kb [ 13]) for map development.
KEAP1 (NM_203500.1) was amplified from EST IMAGE 3163902 and subcloned as a BamHI/NotI insert into a modified pFastbacDualbaculoviral vector containing an N-terminal DAC tag [ 26] with a TEV (tobacco etch virus) protease site downstream of the DAC tag.
The rpoN coding region was amplified with primers pGEXrpoNEcoRIfor and pGEXrpoNXhoIrev, digested with EcoR I and Xho I, and inserted into the EcoR I- Xho I sites of pGEX-4T-1 (GE).
The PCR product was digested with Xba I and Sac I, and inserted into the Xba I and Sac I sites of the pBI121 binary vector between the CaMV35S promoter and the nopaline synthase terminator [49].
The final PCR product was digested with Sfi I and Not I, and inserted into the pPIC-PST vector between Sfi I and Not I sites to produce pPIC- poswo1.
The PCR products were digested with Nde I and BamH I, and inserted into pET-20b at Nde I and BamH I sites, yielding the plasmid pET-BGL.
The PCR products were digested with BamH I and Xho I, and inserted into pET-BGL at BamH I and Xho I sites, yielding the plamid pET-BGL-CBD.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com