Sentence examples for I homing from inspiring English sources

Following amplification in E.coli, the expression cassette from pShuttle was excised using I-Ceu I/PI-Sec I homing enzymes and cloned into an Ad5F35 expression vector.

The transfer construct was engineered within the multiple cloning site (MCS) between the two I-CeuI homing endonuclease restriction sites of plasmid pCeu2.

The first is a HNH endonuclease, a group I homing endonuclease, which may be related to the presence of introns in the UFV-P2 genome [ 30], like those between the two parts of DNA polymerase.

In addition, gp222 present in between gp223 and gp221 in phage Bobb encodes intron endonuclease homolog that contains the N-terminal catalytic domain for GIY-YIG intron endonuclease I-TevI, I-BmoI, I-BanI, I-BthII proteins and a C-terminal YIG family of class I homing endonucleases C-terminus (GIY-YIG_Cterm) (see Discussion for more details).

Mutational analysis of one such RNA secondary structure that regulates translation of the I-TevI homing endonuclease revealed a pronounced splicing defect resulting from ribosome occupancy of intron sequences that form the crucial structures necessary for splicing [ 59].

In addition, pioneering work by Puchta and coworkers has demonstrated that the I-SceI homing endonuclease can be used to excise a selectable marker gene integrated between a pair of I-SceI recognition sites in transgenic tobacco at frequencies ranging from 19 to 75% [ 16].

However, sexual mating is the biological framework for nuclear group I intron homing [ 26, 27] and it is unclear why the apparently asexual Naegleria contains and maintains homing introns.

During group I intron homing, exonic regions immediately flanking the insertion site often engage in a gene conversion process that replaces part of the host exonic sequence and creates a co-conversion tract (CCT) (Mueller et al. 1996; Palmer et al. 2003; Sanchez-Puerta et al. 2011).

I, too, was homing in on the very niche practice of personal-essay writing, though I was far too broke to be discriminating about the work I did for money, which ran the gamut from temporary secretarial work to, as the dotcom era rolled around, "producing content" for a Web site about maxipads.

We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 Å2 protein interface between these domains.

"But if I'm really homing in on the dramatic performance right now, it's probably Christian Bale or it's Joaq".