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(i) Fractionated elution: 87% of 68Ga was recovered in 4.5 min from the collection of the 2-5 mL fractions from both generators.
About 10 µg-purified tail DNA were digested by EcoR I, fractionated by electrophoresis on 0.7% agarose gels and transferred onto Zeta-probe GT membranes (BioRad).
To my surprise, I found that the activity that kept the stem cells undifferentiated separated away from IGF when I fractionated the BRL medium.
Methods: The efficiency of three double elution methods of ITG generators using HCl 0.05M were compared: (i) Fractionated elution: The elution profile of one ITG generator was first determined.
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If contaminated oil (j) from tank (gm) at station (i) is fractionated at time node (t), (Cmf_{i,j,gm}^{t} = 1), if not, (Cmf_{i,j,gm}^{t} = 0).
Then the cDNA was restricted with Sfi I and size fractionated (fraction 1-2.5 kb and >2.5 kb).
One half of HIM-I was fractionated into an ethanol-soluble fraction and into an ethanol-insoluble polysaccharide fraction by a precipitation in 80% ethanol.
When an elementally fractionated (i.e., depleted in He) but isotopically unfractionated component is assumed instead of the normal SW, the isotopic ratios of D10IB130 can be reproduced to some degree.
This pool of sheared DNA fragments was then size fractionated (i.e. fragments between 1.5 3 kb were gel excised), gel purified, and subjected to a series of "polishing" reactions by T4 DNA polymerase and Klenow fragment (Invitrogen, Carlsbad, CA).
Polymerase chain reaction products were digested with the restriction enzyme Nco I and then fractionated by gel electrophoresis.
Full length double stranded cDNAs were digested with Sfi I and size fractionated using a CHROMA SPIN-400 column (CLONTECH, Palo Alto, CA).
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