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Libraries of bacterial chromosomal fragments were screened using two separate systems that monitor: (i) elevated fluorescence conferred by enhanced expression of GPCR GFP fusions and (ii) increased binding of fluorescent ligand in cells producing more active receptor.
Based on these observations, we hypothesised that (i) elevated levels of tCK18 at baseline are associated with poor prognosis, (ii) circulating cCK18 levels are associated with tumour apoptosis following therapy, and (iii) circulating tCK18 correlates with tumour burden in CRC.
Indeed, if related to mock cells, SY5Y-APP clone showed (i) elevated APP mRNA and protein levels, (ii) increased expression of APP metabolites, among them CTFs C99 and C83 and (iii) enhanced A β peptide immunoreactivity.
Anemia in patients with COPD is likely due to a combination of several factors [ 26, 29, 33]: (i) Elevated cytokines levels, especially Tumor Necrosis Factor alpha (TNF α) and interleukin-6 (IL-6) [ 1, 12, 17, 18, 34, 35].
We show that lack of ADAM10 leads to (i) elevated (membrane) levels of PrPC, (ii) drastically shortened incubation times of prion disease, (iii) increased prion conversion, and (iv) upregulation of calpain levels.
Consequently we expected (i) elevated levels of ruminant-specific MST signals when compared to other sources, (ii) a correlation between FIB parameters and ruminant-specific MST signal levels, and (iii) the ability to explain variations in FIB E. coli levels by ruminant-specific quantitative MST signal variations.
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