Sentence examples for I deficient from inspiring English sources

The construct was transfected into the HLA class I deficient human B cell line 721.221 as previously described [37].

MHC class I deficient (B6.129-B2mtm1JaeN12) and MHC class II deficient (B6.129-H2-Ab1tm1GruN12) mice were purchased from Taconic Farms.

In contrast to MHC Class I deficient mice, MHC class II deficient mice already started to die at day 8 post infection and only ∼5% survived (Fig. 5D).

To further investigate the role of T cells in tumor protection we immunized MHC I deficient mice, which lack CD8+ T cells.

Transient expression of cloned MHC class I cDNA was achieved by electroporation of plasmid DNA into the MHC class I deficient human B-cell line 721.221 [66].

These results were confirmed by cloning all three fragments using pCR2.1 as vector, and attempting to transform the DNA polymerase I deficient E. coli C2110.

Genomic engineering resulting in IGF-I deficient mice, and mice with targeted over-expression of IGF-I reinforce the essential role of IGF-I in bone development at both the embryonic and postnatal stages.

In order to determine whether mitochondrial differences occur in cells derived from the low IGF-I environment present in the IGF-I deficient mice, we examined embryo fibroblast cells derived from either IGF-I deficient mice or controls.

We examined tissues from the IGF-I deficient mice for evidence of autophagy by staining for LC-3 puncta.

Flow cytometry analysis revealed that embryo fibroblasts derived from the IGF-I deficient mice had a significantly greater mitochondrial mass than fibroblasts derived from control mice.

We found increased numbers of LC-3 containing puncta in the tissues of the IGF-I deficient mice relative to controls (Fig 7a,b).