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As such, they generally assume that (i) bars are perfectly rigid, (ii) cables are linear elastic, and (iii) bars experience pure compression and strings pure tension.
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The first condition suggests that, in equilibrium, (forall i, bar {i}), where (bar {i}=i+1) or (bar {i}=i-1), (|w_{ji}-w_{jbar {i}}|leq frac {t}{n}).
By Proposition 3.6, we have (bar{u}_{chiOmega_{1}cupOmega_{2}}inmathcal{N}) and (I(bar {u}_{chiOmega_{1}cupOmega_{2}} geqzeta).
Filopodia induction by I-BARs was initially interpreted as a result of their actin-bundling activity [10].
Indeed, I-BARs from several proteins can induce tubular invaginations on membrane vesicles in vitro [11], [13].
I-BARs may be also able to form similar coats as they generate a striated pattern detectable by cryoelectron microscopy when assembled on giant unilamellar vesicles [13].
The scaffolding role of I-BARs is also consistent with the recent analysis of I-BAR-induced filopodia by fluorescence recovery after photobleaching that revealed stable association with the membrane of I-BAR domain from C. elegans, but surprisingly, not of I-BARs from mammalian MIM and IRSp53 proteins, which displayed high dynamics in this assay [13].
The GFP-IRSp53-I-BAR construct has been described [31].
To understand how I-BAR-induced filopodia generate and maintain their shape, we investigated filopodia induced in B16F1 melanoma cells by the GFP-tagged I-BAR domain from IRSp53 (GFP-I-BAR).
Stable cell line expressing GFP-IRSp53-I-BAR was established by FACS and G418 selection.
For this analysis, we used a stable GFP- I-BAR-expressing B16F1 cell line.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com