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Intuitively, new family members should (i) align well and (ii) show the same secondary structure.
We restricted our analysis to genes that: i) align > 50%% to the human ortholog; ii) are represented in the six avian lineages; and iii) show no duplication event within birds, to elude paralogy.
At the gene level, a read can (i) align uniquely to the correct location; (ii) align to multiple locations, one of which is correct; (iii) fail to align to any locus with three or fewer mismatches; (iv) align to multiple locations, none of which are correct; or (v) align uniquely to the incorrect location.
"Everything I do I align with charity," Ms. Lanfrank said.
"If I align with a brand, it needs to align with my values".
I have several groups that I align with…here in NY-CT and my new favorite, the Pasadena Patriots…where they are boots-on-the-ground working to oust Adam Schiff in favor of John Colbert.
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The number of reads x i aligned to genomic position i is an unbiased estimate of RNA abundance.
Finally, a constant gap penalty C f is applied to fusion transitions in which t i aligns to G/ G′ and t j aligns to S/ S′.
The algorithm then iterates the steps of (i) aligning the graphs using the estimated parameters and (ii) estimating the maximum likelihood parameters until convergence.
Later I aligned myself with critics who saw Amanda as the center of things.
I made a decision very early on that unless I aligned the people within the organisation to what we really wanted to do, it was going to be a big challenge to achieve what we had in mind.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com