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I Adhesion of bacteria to biomaterial involves reversible cellular association with the surface.
The collagen I adhesion assay was performed on a collagen I substrate-coated plate as per manufacturer's instructions (Invitrogen, Singapore).
The effect of PEDF on the metastatic potential of osteosarcoma cells was assessed in vitro by collagen I adhesion and invasion assays.
Keeping this in mind, the effects of GnRHR activation on Collagen I adhesion and actin polymerization during this condition were determined.
For instance, LPHN1, a Group I Adhesion, interacts with teneurin-2, FLRT1/3 and neurexin I-alpha & beta, which are all single-pass membrane proteins with a variety of N-terminal domains [ 46- 48].
In this context, the contrasting results on the collagen I adhesion assay between Capan-1 cells and MIA PaCa-2 cells indicate that the de-adhesive response to EGF appears to be a feature characteristic of a metastatic cell, whereas stimulation of adhesion to collagen I by HRG-α appears to be a feature of a primary pancreatic adenocarcinoma cell.
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These data suggested that both α4-integrins mediate OT-I adhesion to the inflamed spinal cord microvasculature.
The major cellular changes comprises: (i) reduced adhesion, spreading and motility on collagen I; (ii) increased apoptosis associated with mitochondrial leakage and Bax upregulation; and (iii) affected integrin-mediated signaling to Akt, FAK and ERK.
Using α2-knockout keratinocytes and dermal fibroblasts, it was shown that α2 integrin regulates the cell attachment and migration on collagen I, whereas adhesion to collagen IV and laminin I is permissive.
Similarly, glycosylation of AIDA-I is required for normal conformation and for AIDA-I mediated adhesion to epithelial cells [39].
Is cell adhesion similar to the adhesion of insects, spiders and geckos?
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