Spots that failed to meet any of the following criteria in either channel were rejected; (i) an intensity value of >300, (ii) signal-to-noise ratio of >3 and (iii) greater than 60% of pixel intensity superior to the median of the local background ± 2 SD.
A I (i, j) represents an intensity value, μ A I i, j and μ B I i, j are intensity means, σ A I i, j and σ B I i, j are intensity standard deviations, and σ A I B I i, j is the intensity covariance.
Two parameters were determined to define signals marking putative small non-coding RNAs: (i) a signal intensity at least two-fold higher than the signal intensity of the background; (ii) at least two significant signals originating from two different probes located in the same intergenic region at a maximum distance of 200 nt.
Signals were classified as small non-coding RNA candidates when exhibiting the following characteristics: (i) a signal intensity at least two fold higher than the background, and (ii) a distance of less than 150 nt between two probes with a positive signal.
Spectra of the biexcitonic (left) and of the excitonic (right) transitions measured for vertical (V) and H polarization (H) for different tuning laser intensities I; where an intensity of I0corresponds to a Rabi frequency of Ω/2Π = 8.3 GHz.
I project an intensity that makes family members uncomfortable".
"At L.S.U., I felt an intensity, maintained through time, that was palpable," said the film director Taylor Hackford, explaining why he set his movie "Everybody's All-American" at the university.
I feel an intensity building up, warmth, fear, excitement, and BOOM.
The method proposed in [30] was used to determine the crystallinity degree (CD) of the samples, in terms of which CD = (I 200 − I am)/I 200 × 100%, where I 200 is an intensity of (200) reflex about 22.5°, and I am is an intensity of amorphous scattering at 18.5°.
There I would work, with an intensity I can recall vividly … I cannot know what I would be doing now had I not cemented my process, as I did there".
The method proposed in [26] was used to determine the crystallinity degree (CD) of the samples, in terms of which CD = (I 200 − I am)/I 200 × 100 %, where I 200 is an intensity of (200) reflex about 23°, I am intensity of amorphous scattering at 18.5°.
