Sentence examples for Hoechst-low cells from inspiring English sources

Side Population (SP) cells, a subset of Hoechst-low cells, are enriched with stem cells.

After 10 events were collected within the live gates, SP and non-SP cells were sorted and defined as Hoechst-low and Hoechst-bright cells, respectively.

Cells that expel the dye are visualized by dual-wavelength flow cytometry (FACS) as a 'Hoechst low' tail of cells, the SP, relative to a larger bulk of 'Hoechst high' cells, the main population (MP).

Cells were incubated with Hoechst33342 (Sigma-Aldrich, Bornem, Belgium) at a final concentration of 5 μg/ml, and the SP was identified as a side branch of 'Hoechst low' cells using dual-wavelength FACS analysis (FACSVantage SE, equipped with FACS DIVA software, version 6.0; BD Biosciences, Erembodegem, Belgium; Hoechst red with 675/20 nm filter and Hoechst blue with 424/44 nm filter).

(C and D) show a rotatosome next to the P. pearti nucleus in fixed and Hoechst stained cells.

Cells were passed through a 70 µm diameter cell strainer and clonally sorted into 96‐well PCR plates by BD FACS Aria II (BD Biosciences) with Hoechst-positive cells.

PI+ cells were expressed as a percentage of Hoechst+ cells.

Hoechst-stained cells were analyzed by fluorescence microscopy.

These findings show that ABCG2/BCRP1 colocalises with Hoechst-dim cells.

Staining with Hoechst visualizes cells with fragmented chromatin.

For Hoechst staining, cells were incubated for 30 min at 28°C with Hoechst at 5 µg.ml−1 final concentration with shaking.