Exact(3)
The arrays were washed with 2× SSC containing 1% SDS 4 times and with 0.1 × SSC containing 0.5%SDS once at 68°C, each for 30 minutes, followed by a final wash with 2× SSC at room temperature for 5 min. The membranes were exposed to BioMax X-ray films with BioMax Transcreen HE intensifying screens (Eastman Kodak Company, Rochester, NY).
The hybridised membrane was then washed four times at 65°C for 30 min in a washing buffer (20 m NaH2PO4 pH 7.2 and 5 mg ml−1 SDS) and exposed to Kodak BioMax MS Film Eastman Kodak Companyy, New Haven, CT, USA) at −80°C for 2 days using BioMax TranScreen HE intensifying screen (Eastman Kodak Company).
The hybridised membranes were exposed for 72 96 h at −80°C using BioMax TranScreen HE Intensifying Screen (Kodak, Rochester, NY, USA).
Similar(57)
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