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Serum samples depleted of exosomes did not have PCR detectable levels of this miRNA.
Because direct PCR sequences are less likely than sequences of molecular clones to have PCR errors [11] and because plasma HIV-1 genomes are more likely than proviral DNA genomes to encode viable viruses [12] we analyzed only direct PCR sequences obtained from plasma HIV-1 isolates.
All 10 assays were found to have PCR efficiencies of greater than 86%.
Unfortunately, the tissue samples from 2004 were lost and so we have PCR data for 2003, 2005, 2006 and 2007.
While carefully designed and optimized to have PCR products with a shorter length (147 201 bp), some of the amplified PCR fragments might form secondary structures (e.g., BA5).
Second, 16S reads that did not have PCR primer sequences at both sequence termini and those with an average quality value < 25 were filtered out.
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In Africa, dihydroartemisinin-piperaquine reduces overall treatment failure compared to artemether-lumefantrine, although both drugs have PCR-adjusted failure rates of less than 5%.
In order to test the possibility that removal of this conserved sequence may alter tissue specificity of the NDUFV1 upstream region, we have PCR-amplified and cloned two genomic fragments, one including the whole, 3665 bp-long upstream sequence, and the other, 3574 bp-long sequence lacking the conserved region.
Thirteen of 18 patients (72%) with clinical stage T3 N0 (IIB) tumors had definitive resections, and 33% had pCR.
And if PCR false positivity is so problematic, then why has PCR emerged as the cornerstone for diagnosing and monitoring a multitude of infectious diseases and where are the warnings from CDC about false positive PCR for these other infectious diseases?
All genes tested had PCR efficiency between 90 and 102%.
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