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He later determined the genetic sequence for the enzyme.
All cDNAs were thoroughly sequenced by Sanger sequencing for verification initially and after all PCR manipulations.
SNP rs55758736 was genotyped by RFLP and sequencing for verification in 178 individuals.
In addition, randomly selected isolates with colonies typical of Staphylococcus hyicus were subjected 16S rRNA gene sequencing for verification.
The PCR products were cloned to a pUCmT vector and sequenced for verification.
All constructs were sequenced for verification.
Recombinant clones were selected and sequenced for verification.
Two or three overlapping regions were PCR amplified to cover the entire viral genome and sequenced for verification.
The SEZ6L2-specific sequence (MHS1011-59266) was obtained from OpenBiosystem Huntsvillee, AL), sequenced for verification, and checked for specificity with BLAST against the human genome.
Clones were fully sequenced for verification.
Each construct was fully sequenced for verification.
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