Thus, following transplantation, 2nd generation tumours were generated with 100% efficiency in both cases (Table 1).
By microinjecting tru-RGN RNAs into zygotes, FVII KO mice were generated with higher efficiency at Site Two (80.1 vs. 35.8%) and Site One (55.0 vs 3.7%) (P < 0.05), but not at Site three (39.4 vs 27.8%) (P > 0.05) when compared with standard RGN controls.
The electric car is superior in many respects when the electricity is generated with an efficiency of 50 60% by a gas turbine engine connected to a high-capacity battery and electric motor.
By using a custom designed fiber-pigtailed periodically poled lithium niobate (PPLN) crystal module the idler beam was generated with an efficiency of 21%/W, yielding 2.6 mW of optical output power.
Using plasmid DNA containing a cloned rRNA gene as template, the upstream (U) and downstream (D) PCR products were generated with similar efficiency (Fig 2A, +p control).
They are probably generated, with differential efficiency, by multiple alternative splicing occurring at the 5′of a single exon.
With the emergence of new, improved sequencing technologies that enable genomic resources to be generated with increasing efficiency and speed, the potential of non-mainstream fish species as disease models can now be explored.
Taken together, these data show that KDR+/CD34+ cells can be generated with equal efficiency across a range of activin A/BMP4 dosing regimens with molecular differences that suggest an endocardial versus vascular endothelial lineage fate (Fig. 3H).
MLPA is a technology based on ligation of multiple probe-pairs hybridized across the entire locus of interest, followed by amplification, facilitated through the use of universal-tag PCR primers for all ligated probe pairs, and subsequently fragment analysis.The use of universal PCR means that amplicons are generated with comparable efficiency and thus the method is semi-quantitative.
The simultaneous measurement of these two transcripts in embryos of both sexes presents, therefore, a challenging situation, because it requires that one amplicon (Oct4) is reliably generated with equal efficiency and sensitivity in the absence as well as in the presence of high amounts of a second amplicon (Xist).
The amplification efficiency was calculated using the equation E = (10−1/slope − 1) × 100%, and only the data generated from reactions with efficiency between 90 and 110% were used for further analysis [ 29].
