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Further, cleavage of SIRT1 by cathepsin triggers stress-induced premature senescence (SIPS) in endothelial progenitor cells (Chen et al., 2012).
Further cleavage to smaller fragments was observed with excess protease.
Further cleavage at Arg generates thrombin and fragment 1·2 (4– 1·2.
Further cleavage of the pre-miRNA by DCLI releases a miRNA/miRNA* duplex.
Further, cleavage products of complement components C3a and C5a are called anaphylatoxins which can be used as biomarkers, if they bind to receptors on mast cells and blood basophils and degranulation.
These particles contain 140-base pair DNA fragments which upon further cleavage with micrococcal nuclease give rise to a pattern of discretely sized DNA fragments characteristic of nucleosome core particles.
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The opening of the ring corresponds to a two-electron mechanism but further cleavages of C O bonds are observable.
This would lead to a decrease of the average length of the peptides products due to further cleavages and thus further shortening of the initial products.
Sixty-minute incubations of Cut-HA protein in increasing concentrations of Ctsl showed a specific production (at 0.4 μg/ml) of near 160 kDa Cut protein isoform, followed by further cleavages at higher protease concentrations.
The observed decrease in apoptotic cell populations after 120-h exposure would be due to early onset of apoptosis followed by further cleavages of DNA fragments that might not be detected by flow cytometry, because the sub-G0/G1 apoptotic cell population was substantially increased to 17.5, 28.5 and 39.4% at 24, 48 and 72 h exposure, respectively (data not shown).
Further, DNA cleavage efficacy of these complexes was investigated by gel electrophoresis.
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